Why should I change to OptiPrep for the isolation of viruses?
Compared to CsCl and sucrose there are procedural advantages to the use of OptiPrep
- OptiPrep is a sterile solution of 60% (w/v) iodixanol; simply dilute it with saline to prepare sterile gradient solutions. It is the only gradient medium manufactured under strict FDA and EU cGMP compliance.
- CsCl and sucrose are toxic to cells and must be removed prior to infection of cells.
- Iodixanol is non-toxic to cells; it has very low endotoxin levels (< 1 EU/ml); measured levels on each batch are usually 0.13 EU/ml. No dialysis is required before re-infection of cells1.
- CsCl must be removed prior to HPLC or gel electrophoresis; iodixanol rarely needs removing prior to further processing.
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